FIG. 2.

Effect of FPN expression on survival of Salmonella in HeLa cells. HeLa cells were transiently transfected with expression plasmids encoding GFP (G), wild-type FPN (F), or the Q182H mutant of FPN (Q). The results of Western blotting for FPN and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) expression are shown at the top. The lower part shows the number of intracellular Salmonella surviving at 2 or 20 h postinfection. Means (plus standard deviations; n = 3) from a single experiment are shown. The results are representative of two such experiments for the GFP expression construct and at least five such experiments for the FPN and Q182H mutant constructs.