Figure 1.

Immunocytochemical detection of cell surface macromolecules expressed on stratified squamous epithelium and reticulated cryptal epithelium in human palatine tonsil. Tissue sections were incubated with primary antibodies as indicated below. Positive cells were identified with biotinylated secondary antibodies and streptavidin-peroxidase conjugates and are stained brown. All sections were counterstained with hematoxylin. a: cytokeratin-stratified squamous epithelium; b: cytokeratin-cryptal epithelium; c: control mouse antibody; d: HS; e: GalCer-cryptal epithelium; f: S100-dendritic cell marker; g: CD3; h: CXCR4; i: CCR5; j: CXCR4 – showing variability in the distribution of CXCR4 positive cells and the reduced thickness of stratified epithelium overlying a follicle at the lower left side. Note that in this large stretch of epithelium, an occasional CXCR4⁺ cell may be a dendritic cell but based on Fig 1f, the overall abundance of dendritic cell is very low. Original magnification a-i: ×400; j: ×100.