Fig. 6.

Nox 4 in HUVEC mediates TGF-β-induced ROS production. A: HUVEC were transduced with adenovirus encoding lacZ (a, b), or dominant-negative mutant Nox4 lacking NADPH binding site (c, d). Cells were loaded with DCF and treated with TGF-β (10 ng/ml, 5 min) and imaged using live confocal microscopy. Representative photographs were taken from 3 separate experiments. B: to quantitate ROS production, the Amplex Red assay was used to measure H₂O₂ production in HUVEC following TGF-β treatment alone and in the presence of DPI, LacZ adenovirus, and dominant-negative mutant Nox4. There were 6 replicates per condition and 3 separate experiments were performed. Data are presented as the mean percent control of H₂O₂ production ± SE from a representative experiment. ^*P < 0.05 vs. control.