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Influence of ligands and the MalTc26 substitution on the proteolysis of MalT by proteinase K. Mixtures were digested for 15 min. (A) Role of the adenine nucleotide. Proteinase K/MalT ratio: 1:64 (samples with maltotriose) and 1:128 (samples without maltotriose). (B) Proteolysis of the MalTc26 variant by proteinase K. Proteinase K/MalTc26 ratio: 1:64 (samples with maltotriose) and 1:128 (samples without maltotriose).
