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Affinity chromatography of ATP on an Ni-NTA DT1H or DT3 column. ●, ■, DT1H column; ○, □, DT3 column; ○, ●, protein concentrations; □, ■, ATP concentrations. The abscissa corresponding to the flow-through obtained at the first spin in the presence of 400 mM imidazole is indicated by the arrow.
