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The effect of mutations in Rpp20 on its ATPase activity
| Enzyme | Relative activity, % |
|---|---|
| Wild type | 100 |
| Mutants in the DIxxN box | |
| D36E | 7 |
| D36A | 110 |
| N40Q | 25 |
| Mutants in the ABC signature motif | |
| D57A | 94 |
| G59A | 89 |
| G59S | 0 |
| R61A | 44 |
The activities of Rpp20 and its mutant derivatives are shown as percentages of the wild-type activity, which is about 65 nmol/min per mg. Each value represents the average of two independent determinations with a variation about 10%.
