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Oxidation of the blue complex by H2O2. Reaction mixtures contained 50 mM Hepes buffer (pH 7.55), 400 μM Fz, 60 μM FeSO4, and no (A), 8 (B), or 80 (C) mM histidine. Immediately after each spectrum was first recorded, 30 mM H2O2 was added and the spectrum was recorded again.
