Figure 6.
Effect of Fe, histidine, and Fz concentrations and pH on blue compound formation and its subsequent conversion to purple and colorless products. Concentrations of reactants were as follows: (A and E) 150 μM Fe(II), 150 μM Fz, and histidine as indicated; (B and F) 150 μM Fe, 150 mM histidine, and Fz as indicated; (C and G) 150 μM Fz, 150 mM histidine, and Fe(II) as indicated; (D and H) 100 μM Fz, 100 μM Fe(II), 100 mM histidine, and pH varied as indicated. □, initial blue; ○, % purple compound; ●, % colorless products.
