Fig. 4.

Fluoxetine increases NB1 cells in the adult DG. (A and B) Immunostaining for PSA-NCAM (green), and nestin-CFPnuc (red). Two cell types are distributed throughout the SGZ, often in close apposition to each other; however, they do not overlap, as illustrated in B (PSA-NCAM cell is red, and nestin-CFPnuc nuclei are green; note that colors are switched at low magnification for better visualization). (C–G) Postmitotic precursors in the fluoxetine-treated DG of adult mice, analyzed 1 day after BrdU labeling. Fluoxetine increases the number of NB1 (C) but not of more advanced NB2 (D) or IN (E) cells. V, vehicle; F, fluoxetine. n = 8 per group. ∗∗, P < 0.01. F and G are representative photomicrographs of DG from control (injected with vehicle) (F) and fluoxetine-treated (G) animals. (Scale bars: A, 20 μm; B, 5 μm; F and G, 10 μm.)