Figure 3.

The effect of egl-4 activity on intestinal darkness and fat storage. (A) The average intestinal grayscale value of six to eight worms subtracted from a maximum grayscale value of 256 and divided by this average value calculated for wild-type worms. A higher value means a darker intestine. Error bars represent standard deviations. To assess for statistical significance of the effect of the n479 and ad450sd mutations on intestinal darkness, six pairwise one-way ANOVAs were performed as marked above the bars. An asterisk indicates statistical significance at P < 0.05 and NS indicates not significant. (B) Shown are six to eight adult worms viewed under a Rhodamine fluorescence filter after cultivation on agar plates containing Nile red. Arrows point to the anterior part of the intestines, where staining appears brightest. Three different observers who were blinded to genotype identified ad450sd as having the brightest staining and n479 as having the faintest staining among these three genotypes.