Figure 5.

Morphological defects caused by the ΔatmA mutation. Wild-type hyphae grown on YG (A) or M-glycerol (B) media. ΔatmA mutant grown on YG (C and D) or M-glycerol (E) media. Note the zig-zag or curled hyphae in C and D and the failure to establish polarity in E. Wild-type (F) and ΔatmA (G) hyphae growing on the surface of a YG plate. Note that wild-type hyphae radiate outward in a linear fashion, whereas mutant hyphae do not and frequently curl backward. (H) Germination pattern of wild-type and ΔatmA conidiospores. Spores were allowed to germinate on YG media for 11 hr. Spores possessing two germ tubes were classified as displaying (left to right) bipolar, quarterpolar, or random germination patterns. N = 200. Bar, 10 μm.