Figure 3.

Multicopy suppression of git2⁻ mutations by plasmid-expressed git1⁺. Host strains CHP7 (git2-7), CHP61 (git2-61), and CHP216 (git2-216) were transformed to Leu⁺ with pNMT41 (empty vector) or pRSK3 (git1⁺). Four independent transformants of each host strain and plasmid combination indicated in the figure were plated onto EMM–leu and then replica plated after 1 day to either EMM–leu or 5-FOA media. 5-FOA plates were photographed after 5 days at 30°. All transformants grew equally well on EMM–leu medium (not shown). β-Galactosidase activity was determined as described in materials and methods. The values given represent specific activity ± standard deviation from three independent transformants.