Figure 7.

git1Δ cells display defects associated with the loss of cAMP signaling. (A) Homothallic (h⁹⁰) strains CHP795 (git1⁺) and CHP896 (git1Δ) were grown to exponential phase in PM liquid medium (at 37° to inhibit conjugation) and then diluted to 10⁶ cells/ml in PM liquid medium in the presence or absence of 5 mm cAMP. These cells were incubated for 24 hr at 30° without shaking and photographed. (B) Strains 972 (git1⁺) and CHP973 (git1Δ) were grown to exponential phase in YEL. Cells displaying septa were measured and photographed.