Figure 8.

Git1 and Git2 (adenylate cyclase) physically interact. (A) Immunoblot of protein extracts from α-V5 immunoprecipitation. Protein extracts were prepared from strains expressing tagged (Git2-myc and Git1-V5) or untagged proteins (+) as indicated. Crude extracts, along with fractions that bound or failed to bind α-V5 antibodies, were probed with α-myc to detect Git2-myc and with α-V5 to detect Git1-V5. (B) Immunoblot of protein extracts from α-myc immunoprecipitation. Protein extracts were prepared from strains expressing tagged or untagged proteins as indicated. Crude extracts, along with fractions that bound or failed to bind α-myc antibodies were probed with α-V5 to detect Git1-V5 and with α-myc to detect Git2-myc.