TABLE 2.
Glucose repression of fbp1-lacZ expression in transformants
| Strain (relevant genotype) | Plasmid (expressed gene) | β-Gal activity |
|---|---|---|
| FWP72 (git1+) | pNMT41 (none) | 10 ± 1 |
| CHP568 (git1-1) | pNMT41 (none) | 1313 ± 16 |
| CHP568 (git1-1) | pRSK3 (git1+) | 37 ± 6 |
| CHP568 (git1-1) | pRSK4 (git1-V5his6) | 31 ± 4 |
| CHP568 (git1-1) | pRSK8 (git1ΔC2) | 729 ± 32 |
| FWP72 (git1+) | pRSK8 (git1ΔC2) | 47 ± 10 |
β-Galactosidase activity was determined from at least two independent transformants as described in materials and methods. The values represent the average ± standard deviation and are given as specific activity per milligram of soluble protein.