TABLE 3.
Defect in glucose repression of fbp1-lacZ expression in mutant strains
| β-Galactosidase activity
|
|||
|---|---|---|---|
| Strain | Relevant genotype | 8% glucose | 8% glucose + cAMP |
| FWP72 | Wild type | 15 ± 8 | ND |
| RKP2 | git1Δ | 834 ± 128 | 12 ± 2 |
| RKP15 | SPAC11E3.02cΔ | 13 ± 5 | ND |
| RKP62a | git1Δ SPAC11E3.02cΔ | 776 ± 81 | ND |
| CHP889 | gpa2R176H | 18 ± 10 | ND |
| RKP9 | git1Δ gpa2R176H | 705 ± 123 | ND |
Cells were grown in YEL to exponential phase under repressing conditions (8% glucose) and assayed as described in materials and methods. The values given represent specific activity ± standard deviation from three or four independent cultures of each strain. ND, not determined.
a
RKP62 values are based on an average of assays of strains RKP62, RKP66, and RKP67, which are genetically identical.