TABLE 1.
Plasmids used in this study
| Plasmid | Auxotrophic marker | Promoter | Copy number | Source |
|---|---|---|---|---|
| pRS416GPD-HSF1 | URA3 | GPD | CEN, low | This study |
| pRS314-HSF1 | TRP1 | HSF1 | CEN, low | Morano et al. (1999) |
| pRS314-HSF1ΔNTA(148–833) | TRP1 | HSF1 | CEN, low | This study |
| pRS314-HSF1ΔCTA(1–583) | TRP1 | HSF1 | CEN, low | Morano et al. (1999) |
| pCUP1-GFP | URA3 | CUP1 | CEN, low | Lindquist lab |
| pCUP1-Sup35GFP | URA3 | CUP1 | CEN, low | Lindquist lab |
| pCUP1-NMGFP | URA3 | CUP1 | CEN, low | Lindquist lab |
| pCUP1-Rnq1GFP | URA3 | CUP1 | CEN, low | Sondheimer and Lindquist (2000) |
| pCUP1-Ure2(1–65)-GFP | URA3 | CUP1 | CEN, low | Lindquist lab |
| pRS313CUP1-NMGFP | HIS3 | CUP1 | CEN, low | Derkatch et al. (2001) |
| pRS305-HSP104 | LEU2 | HSP104 | Integrating, single | This study |
| pRS415-HSP104 | LEU2 | HSP104 | CEN, low | This study |
| p425GPD-HSP104 | LEU2 | GPD | 2μ, high | This study |
| P426/PQ25 | URA3 | GPD | 2μ, high | Krobitsch and Lindquist (2000) |
| P426/PQ47 | URA3 | GPD | 2μ, high | Krobitsch and Lindquist (2000) |
| P426/PQ72 | URA3 | GPD | 2μ, high | Krobitsch and Lindquist (2000) |
| P426/PQ103 | URA3 | GPD | 2μ, high | Krobitsch and Lindquist (2000) |