Skip to main content
. 2001 Jan 2;98(2):497–501. doi: 10.1073/pnas.240461397

Figure 3.

Figure 3

RL-translated cyclin B protein is not required to induce cyclin A cleavage. (A) Blocking cyclin B synthesis does not affect cyclin A cleavage. Cyclin B DNA was mixed with RL either in the absence or presence of cycloheximide (1 mg/ml). No cyclin B translation was detected when cycloheximide was added (Lower). The RL were then incubated with GST-cyclin A(CΔ114) for the indicated time, and cleavage of cyclin A was detected by SDS/PAGE and Coomassie blue staining (Upper). (B) Cyclin A proteolytic activity is present in the DNA and DN5α lysate. GST-cyclin A(CΔ114) was incubated with buffer, cyclin B(RL), cyclin B DNA, DH5α lysate, or BL21(DE3) lysate as indicated. GST-cyclin A(CΔ114) was not added in the reactions in lanes 6 and 8. Cleavage of cyclin A was detected by SDS/PAGE and Coomassie blue staining. Molecular size standards (lane 1) in kDa are indicated on the left.