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. 2001 Jan 2;98(2):497–501. doi: 10.1073/pnas.240461397

Figure 6.

Figure 6

Cleavage of cyclin A by OmpT. (A) GST-cyclin A(CΔ114) was incubated with buffer (lanes 2 and 3), lysates of BL21(DE3) (lane 4), BL21(DE3) transformed with cyclin B construct (lane 5), or transformed with OmpT expression construct (lane 6) for the indicated time. Cleavage of cyclin A was detected by SDS/PAGE and Coomassie blue staining. Molecular size standards (lane 1) in kDa are indicated on the left. (B) Purified OmpT protein (lanes 4–6) was incubated with GST-cyclin A(CΔ114) (lanes 2–5) for the indicated time. Cleavage of cyclin A was detected by SDS/PAGE and Coomassie blue staining. Molecular size standards (lane 1) in kDa are indicated on the left.