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. 2001 Jan 9;98(2):507–512. doi: 10.1073/pnas.021488798

Figure 1.

Figure 1

Coordinate induction of apoE, ABCA1, and ABCG1 expression in THP-1 macrophages by modified LDL. Differentiated THP-1 macrophages were incubated for 48 h in RPMI medium 1640 containing 10% (vol/vol) LPDS with mevinolin and mevalonic acid, and 100 μg/ml (protein) LDL, highly oxidized LDL, or acetylated LDL, as indicated. Total RNA (10 μg per lane) was electrophoresed through formaldehyde-containing gels, transferred to Nylon, and hybridized to 32P-labeled cDNA probes. 36B4 was used as a control (ctrl) for loading and integrity of the RNA.