Skip to main content
. 2001 Jan 9;98(2):507–512. doi: 10.1073/pnas.021488798

Figure 3.

Figure 3

Macrophage- and adipocyte-specific induction of ApoE expression by LXR and LXR ligands. (A) Differentiation-dependent induction of apoE expression by LXR ligands. Undifferentiated (monocyte) or differentiated (macrophage) THP-1 cells were incubated in RPMI medium 1640 containing 10% (vol/vol) LPDS and 2.0 μg/ml 22(S)HC, 2.0 μg/ml 20(S)HC, 2.0 μg/ml 22(R)HC, or vehicle control (ctrl) for 48 h. (B and C) Retroviral expression and activation of LXRα induces apoE expression in preadipocytes but not in fibroblasts. NIH 3T3 fibroblasts and 3T3-F442A preadipocytes were transduced with a retroviral vector encoding LXRα (NIH-LXRα, 442A-LXRα) or the empty vector alone (NIH-vector, 442A-vector). Stable cell lines were cultured for 48 h in DMEM containing 10% (vol/vol) LPDS in the presence of 50 nM LG268 (LG), 2.0 μg/ml 22(R)HC, or vehicle control (ctrl). Northern analysis was performed as described above.