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. 2001 Jan 9;98(2):507–512. doi: 10.1073/pnas.021488798

Figure 6.

Figure 6

Tissue-specific induction of apoE expression in vivo by LXR ligands. (A) Sterol-induced expression of apoE in macrophages requires LXR. Peritoneal macrophages were isolated as described in Materials and Methods and cultured for 42 h in the presence of 100 μM mevalonic acid (control) or mevalonic acid plus 5 μM compactin (unloaded), 10 μM 22(R)-hydroxycholesterol [22(R)HC], or 10 μM T0901317. (B and C) LXR and RXR ligands induce apoE expression in an LXR-dependent manner in adipose tissue (B) but not in liver (C). Wild-type or Lxrα/β−/− mice were fed ad libitum diets containing 0.2% cholesterol and vehicle (Veh), LXR agonist (T0901317, 50 mg/kg body weight) or RXR-specific agonist (LG268, 30 mg/kg body weight) for 10 days. Northern analysis was performed as above. Blots were quantitated by PhosphorImager, standardized against actin or cyclophilin (cyclo), and mathematically adjusted to establish a unit of 1 for the wild-type group receiving vehicle.