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Effect of AAF-cmk on AAF-amc hydrolysis in EL4ad cells. EL4ad cells were treated with 10 μM AAF-cmk or 10 μM lactacystin for 90 min at 37°C and then incubated with 100 μM AAF-amc. Hydrolysis of AAF-amc was determined by measuring fluorescence at 5–10-min intervals.
