Table 1.
Inhibitor concentrations resulting in 80% growth inhibition of EL4 and EL4ad cells
Inhibitor | EL4, μM | Fold difference inhibitor
concentration
|
|
---|---|---|---|
EL4ad + 50 μM NLVS | EL4ad | ||
Lactacystin | 4 | 0.75 × | 1.25 × |
zLLL | 0.4 | 1 × | 3.75 × |
NLVS | 8 | >6 × | >6 × |
Epoxomicin | 0.03 | 23 × | 50 × |
Boro-LLL | 0.04 | 12.5 × | >25 × |
PS-341 | 0.02 | 1 × | 2 × |
YU101 | 0.25 | 4 × | 8 × |
AAF-cmk (Sigma) | 20 | 1 × | 1.25 × |
AAF-cmk (Bachem) | 20 | 1.5 × | 1.5 × |
Cells were cultured in RP10 with varying concentrations of the indicated inhibitors, and the number of viable cells was determined by trypan blue exclusion every 24 h. After 72 h in culture, the concentration of inhibitor necessary to cause an 80% reduction in the number of viable cells relative to control cultures was determined. The fold difference in inhibitor concentration for EL4ad relative to EL4 cells is indicated.