Table 3.

Estimates of nucleotide diversity and test statistics for selection at “adh3” and adh1 in wild barley (length of the regions is the average number of bases excluding alignment gaps for all lines)

Gene	Region	Length, bp	θ/bp	π/bp	T	D*	F	F*
“adh3” whole sample
(n = 25 accessions)
	All	1,842.8	0.0149	0.0219	1.734†	0.348	0.931	0.858
	Intron	921.9	0.0161	0.0257	2.183‡	0.929	1.599	1.437
	Exon	929.1	0.0137	0.0179	1.127	−0.350	0.073	0.127
	Rep.	715.0	0.0082	0.0088	0.279	−0.746	−0.588	−0.462
	Syn.	212.1	0.0325	0.0488	1.759†	0.038	0.636	0.621
“adh3” cluster 1
(n = 13 accessions)
	All	1,836.6	0.0049	0.0030	−1.544†	−1.843†	−2.367‡	−1.835†
	Intron	917.4	0.0039	0.0029	−0.890	−1.096	−1.378	−1.083
	Exon	920.8	0.0059	0.0031	−1.847‡	−2.153†	−2.706‡	−2.154†
	Rep.	703.8	0.0050	0.0028	−1.666†	−1.790†	−2.256‡	−1.826†
	Syn.	212.4	0.0091	0.0043	−1.755‡	−2.221‡	−2.585‡	−2.178‡
“adh3” cluster 2
(n = 12 accessions)
	All	1,864.7	0.0023	0.0024	0.094	0.492	0.385	0.402
	Intron	926.8	0.0018	0.0017	−0.130	0.513	0.338	0.359
	Exon	938.0	0.0028	0.0030	0.232	0.390	0.330	0.361
	Rep.	721.8	0.0023	0.0025	0.357	0.513	0.476	0.489
	Syn.	217.2	0.0046	0.0045	−0.025	0.078	−0.012	0.053
adh1
(n = 25 accessions)§
	All	1,359.9	0.0029	0.0021	−0.926	−1.705	−1.828†	−1.582
	Intron	574.2	0.0032	0.0027	−0.436	−0.674	−0.747	−0.648
	Exon	786.0	0.0027	0.0016	−1.174	−2.169†	−2.249†	−2.013†
	Rep.	606.4	0.0022	0.0007	−1.858‡	−2.890‡	−3.022‡	−2.776‡
	Syn.	179.6	0.0044	0.0049	0.247	−0.192	−0.109	−0.071

θ, Watterson's estimate; π, Tajima's estimate; T, Tajima's D test; D*, Fu and Li's D test without outgroup information; F, Fu and Li's F test; F*, Fu and Li's F* test. 

^† 0.01 < P < 0.05. 

^‡ P < 0.01. 

^§ adh1 data from ref. 10.