Figure 2.

Amino acid alignments of vertebrate PU.1 genes. The predicted amino acid sequence of skate PU.1 is aligned with PU.1 sequences from mouse, human, chicken, and teleost fish by using clustalw (see Fig. 1 legend for GenBank accession nos.). Stars indicate identity in all sequences, and dots indicate conserved types of amino acids. The transactivation domain is divided into four functionally relevant subdomains indicated above the sequence. The colored amino acids have been shown to be necessary for mouse PU.1 transactivation activity; Ser-148 is pink.