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. 2006 Apr 11;395(Pt 3):463–472. doi: 10.1042/BJ20051728

Figure 1. Thermal stability of reduced and oxidized Ara h 2.

Figure 1

Far-UV CD spectra of Ara h 2 (10 μM) in the absence (A) and presence (B) of 1 mM DTT at 20 °C (●), 90 °C (○) and 20 °C (○) after cooling in 10 mM potassium phosphate buffer (pH 8). A 1 mm pathlength was used with a scanning speed of 20 nm/min, 6-fold accumulation rate, corrected for buffer effects. Denaturation (○) and refolding (●) of Ara h 2 (1.5 μM) in 10 mM potassium phosphate buffer (pH 8) in the absence (C) or presence (D) of 0.5 mM DTT. Transitions were monitored with a CD signal at 222 nm with a 1 cm pathlength and heating rate 1 °C/min. deg, degrees.