Table 2. Hydrogen bonds around the catalytic triad (Asp32, His64 and Ser221) and oxyanion hole (Asn155) of NK.
Proton-supplying group | Proton-accepting group | ||||
---|---|---|---|---|---|
H-bonds | Residue | Atom | Residue | Atom | Bond length (Å) |
1 | Asp32 | HN | Val93 | O | 2.2±0.2 |
2 | Ser33 | HN | Asp32 | Oδ1 | 1.8±0.1 |
3 | Ser33 | HOγ | Asp60 | Oδ1 | 1.7±0.1 |
4 | Gly61 | HN | Ser33 | O | 2.0±0.3 |
5 | Ser62 | HOγ | Ser33 | Oγ | 2.0±0.3 |
6 | Ser62 | HN | Asp60 | Oδ1 | 2.7±0.7 |
7 | Ser63 | HN | Asp60 | Oδ1 | 2.7±0.7 |
8 | His64 | HNe1 | Asp32 | Oδ1 | 1.8±0.1 |
9 | His64 | HNe1 | Asp32 | Oδ2 | 2.7±0.5 |
10 | His64 | HN | Ser62 | Oγ | 2.3±0.5 |
11 | Thr66 | HN | Asp60 | Oδ2 | 1.9±0.2 |
12 | Thr66 | HOγ | Asp60 | Oδ2 | 1.8±0.2 |
13 | Val68 | HN | His64 | O | 2.2±0.3 |
14 | Val95 | HN | Asp32 | O | 2.0±0.2 |
15 | Thr220 | HOγ | Asn155 | Oδ | 2.4±0.5 |
16 | Thr220 | HN | Asn155 | Oδ | 2.0±0.2 |