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. 2006 Apr 11;395(Pt 3):529–535. doi: 10.1042/BJ20051646

Figure 3. Mapping of the major proteolytic fragments of BRCA1-(230–534), resulting from limited trypsinolysis, by MALDI-TOF.

Figure 3

(a) Mass spectrum of peak 62. The heterogeneity observed in the peaks is due to cleavage at two adjacent lysine residues and a proportion of the sample retaining the N-terminal methionine. (b) The amino acid sequence of BRCA1-(230–534). The highlighted region was identified as a domain produced from limited trypsinolysis. This region has a calculated molecular mass of 12556 Da and thus domain boundaries are identified as residues 230–339.