Table 1. Primer sequences used to amplify BRCA1 protein fragments.

For each pair, the first sequence is the N-terminal primer and the second is the C-terminal primer.

Construct	PCR primers
BRCA1-(230–347)	5′-TGCCGACATATGGAGACGCATCTAACAAATAC-3′
	5′-ACTCCGAAGCTTTCACTCGAGCAGGGGATCAGCATTCAGATCTACC-3′
BRCA1-(340–513)	5′-TGCCGACATATGGTAGATCTGAATGCTGATCCC-3′
	5′-ACTCCGAAGCTTTCACTCGAGAAGGCCTGATGTAGGTCTCC-3′
BRCA1-(340–554)	5′-TGCCGACATATGGTAGATCTGAATGCTGATCCC-3′
	5′-ACTCCGAAGCTTTCACTCGAGCTCTAGACCACTATTAGT-3′
BRCA1-(340–595)	5′-TGCCGACATATGGTAGATCTGAATGCTGATCCC-3′
	5′-ACTCCGAAGCTTTCACTCGAGTTCCATATTGCTTATACTGCTGC-3′