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. 2006 May 15;396(Pt 2):219–226. doi: 10.1042/BJ20060108

Figure 5. Cleavage kinetics of PABP, eIF4GI and eIF4GII in vitro by purified recombinant HIV-1 and HIV-2 proteases.

Figure 5

Crude HeLa S10 extracts (50 μg) were incubated with 3 ng/μl HIV-1 protease (A) or HIV-2 protease (B) in a total volume of 20 μl during the indicated times and subjected to Western blot analysis by using a monoclonal antibody against PABP (top panel; 10% gel), a mixture of antisera against N-terminal and C-terminal regions of eIF4GI (middle panel; 7.5% gel) or an antiserum against the C-terminal region of eIF4GII (bottom panel; 7.5% gel). Degradation kinetics of PABP, eIF4GI and eIF4GII in HeLa cell extracts incubated with HIV-1 protease (C) and HIV-2 protease (D). Determinations were obtained by densitometric scanning of the corresponding intact protein band at the indicated times. MW, molecular mass (sizes given in kDa).