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. 2006 May 15;396(Pt 2):327–336. doi: 10.1042/BJ20051391

Figure 2. Functional analysis of various 5′-deletion constructs in C2BBe1 cells in the presence and absence of PMA.

Figure 2

(A) A schematic representation of the NHE3 proximal promoter region and the location of the potential transcription-factor-binding sites are shown. (B) 5′-Deletion constructs were transiently transfected into C2BBe1 cells. Cells were serum-starved in 0.5% serum for 24 h prior to addition of PMA (100 nM) for 16 h (PMA-induced NHE3 promoter activity) or kept in serum-starvation media for the same duration (basal NHE3 promoter activity). Transfected cells were harvested 48 h post-transfection and cell lysates were prepared as described in the Experimental section. Luciferase activity was measured and normalized to β-galactosidase activity as an internal control for variations in transfection efficiency and presented relative to the normalized activity of the promoter-less pGL2-Basic. Error bars represent the S.E.M. (n=3). AP-2, activator protein-2; TFIID, transcription factor IID complex.