Figure 8. PMA-induced hNHE3 promoter activity is PKC-independent.

C2BBe1 cells were transiently transfected with 5′-truncated NHE3 promoter-reporter constructs and incubated in serum-reduced media at least 24 h prior to treatments. Cells were either not treated or pretreated with the PKC inhibitor chelerythrine chloride (2 μM) for 60 min prior to the addition of PMA (100 nM). As control 4-α phorbol ester (100 nM) was used to serve as inactive PMA. After 16 h of PMA treatment, cells were harvested and luciferase activities were determined. Results are presented as the means±S.E.M. (n=3–5). *The treatment pair PMA and PMA+chelerythrine chloride was analysed with unpaired t test and showed no significant differences.