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. 2006 May 15;396(Pt 2):363–369. doi: 10.1042/BJ20051478

Figure 3. SNAP-25 and S180 bind Kv2.1 cytoplasmic domain proteins.

Figure 3

Binding assays were performed using GST, GST–Kv2.1-N, –C1, or –C2 fusion proteins (all bound to glutathione–agarose beads, 450 pmol of protein) to pull-down overexpressed SNAP-25-WT or S180 from an HEK-293 cell lysate extract. (A) Top panel shows a representative Western blot of the precipitated SNAP-25 (lanes 1–4) and S180 proteins (lanes 5–8). The HEK-293 cell lysate extract-1 contained overexpressed SNAP-25-WT protein (lane 9), and -2 contained overexpressed S180 protein (lane 10). Bottom panel, a summary of the quantitative densitometric scanning of blots from three separate experiments. The values (means±S.E.M.) are expressed as a percentage of the maximum. (B) Representative Ponceau S staining of the blot shows that equal amounts of GST proteins were loaded.