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. 2006 May 15;396(Pt 2):215–218. doi: 10.1042/BJ20060372

Figure 3. Activation of AC by CO2.

Figure 3

(a) Ratio of the specific activities of Slr1991120–337 when assayed in the presence of 40 mM total Ci or NaCl at various pH values (8 μM protein, 1 mM Mg2+-ATP and 20 mM Mg2+). The inset shows the percentage of total Ci made up by CO2 and HCO3− over the pH range tested. (b) Change in pH of a 5 mM Mes solution (starting pH 6.4) on addition of 20 mM NaHCO3 (↑) in the presence (□) or absence (△) of 132 units of carbonic anhydrase at 0 °C. (c) cAMP produced by Slr1991120–337 under conditions of Ci disequilibrium (50 μM Slr1991120–337 protein, 0 °C, 10 s, 20 mM CO2/NaHCO3/NaCl, 100 mM Mes, pH 6.5, 150 μM Mn2+-ATP). (d), cAMP produced by CyaB1595–859 under conditions of Ci disequilibrium (38 μM CyaB1595–859 protein, 0 °C, 10 s, 20 mM CO2/NaHCO3/NaCl, 100 mM Mes, pH 6.5, 150 μM Mn2+-ATP, n=6).