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. 2006 May 18;116(6):1535–1546. doi: 10.1172/JCI25442

Figure 1. Generation and characterization of epimorphin–/– mice.

Figure 1

(A) Targeting scheme to disrupt the epimorphin locus. Homologous recombination between the targeting vector and the WT allele produced the targeted allele in which 30 bp of the coding sequence in exon 1 and 1015 bp in intron 1 of the epimorphin gene were replaced by the coding sequence of enhanced GFP and a Neo cassette. The black bar represents the location of the probe used to identify targeted ES cell clones. The arrows denote the position of the initiator methionine of epimorphin and GFP. (B) Southern blot hybridization of Hind III–digested genomic DNA. A 10.4-kb band in the targeted allele and 8.7-kb band in the WT allele were produced as expected. (C) Immunoblot analysis of protein extracts from epimorphin WT (+/+), heterozygous (+/–), and null (–/–) mice. Total protein from small intestine, colon, liver, kidney, and testis (100 μg per tissue) was loaded onto the gel. Epimorphin was reduced in the tissues of heterozygous mice and absent in null mice.