Fig 1.

Corticotropin-releasing hormone receptor type 1 selective agonist (continuous curve, square points) and urocortin I (dotted curve, circle points) attenuate proliferation of HaCaT cells. HaCaT cells were stimulated for 48 h in supplement-free low-calcium EpiLife medium with peptides at graded concentrations. Cell viability was estimated with a MTT test. The results are presented as mean ± SEM (n = 16). Fitting of the dose–response curve was performed with the Prism 4·0 software (GraphPad, San Diego, CA, U.S.A).