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. Author manuscript; available in PMC: 2006 May 17.
Published in final edited form as: Ann N Y Acad Sci. 2005 Nov;1058:105–118. doi: 10.1196/annals.1359.019

Fig. 1.

Fig. 1.

RNAi mediated by synthetic siRNA duplexes depends on active cell growth. Efficiencies of synthetic p53 siRNA and non-specific (NS) siRNA duplexes on cellular p53 expression were assayed in CaSki cells at 2 × 105 at 20-24 hours (0 time) after plating by transient transfection by using oligofactamine (Invitrogen). Cell samples were prepared at 24, 48, 72, 96, and 120 hours after transfection of each siRNA (20 nM) as diagramed above and examined by Western blot for p53 by using anti-p53 antibody (Oncogene). Cells with (lane 7) or without (lane 6) re-transfection with either NS or p53 siRNAs at 96 hours of the initial siRNA transfection, as indicated in diagram above, were further compared and showed no difference in responding to p53 siRNAs at 120 h post the initial transfection.