Figure 2.

Complex transcription factor choreography precedes FBP and FIR recruitment to the c-myc promoter. Human Hs68 primary fibroblasts were cultured in DMEM without FBS for 5 days and then stimulated with 10% serum; cells were harvested for RNA or ChIP at the indicated times after adding serum. (A) RNase protection assay measuring c-myc levels. (B, C) Chromatin from starved and stimulated cells was immunoprecipitated with indicated antibodies. (D) Real-time PCR amplification of the same ChIP DNA samples shown in (C) (2 and 4 h). (E) Schematic representation of transcription factor binding sites and primer sets for PCR amplification.