Skip to main content
. 2006 Apr 20;25(10):2240–2252. doi: 10.1038/sj.emboj.7601099

Table 1.

Pheromone does not induce shmooing in white cells or block them in G1

          Percent of population
Cell type Growth phasea Growth time (h)b Pheromone treatmentc No. of cells Unbudded cells (%) Cells with bud (%) Shmoos (%)
White ESP 0 276 70 30 0
    8 289 17 83 0
    8 + 213 7 93 0
               
White LSP 0 206 97 3 0
    8 204 5 94 0
    8 + 214 6 94 0
               
Opaque ESP 0 258 76 25 0
    8 224 22 78 0
    8 + 200 9 4 85
               
Opaque LSP 0 221 86 13 0
    8 194 15 85 0
    8 + 206 9 19 72
aESP, early saturation phase; LSP, late saturation phase.
bWhite or opaque cells were grown at 25°C in supplemented Lee's medium for 36 h to early saturation phase or for 48 h to late stationary phase, and diluted into fresh medium at 2 × 106/ml. Cells were then incubated for 8 h.
cFor pheromone-treated cells (+), pheromone was added every hour for 7 h, then cells were harvested at 8 h. Untreated (−) cells were also harvested at 8 h.