Table 1.
Pheromone does not induce shmooing in white cells or block them in G1
Percent of population |
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---|---|---|---|---|---|---|---|
Cell type | Growth phasea | Growth time (h)b | Pheromone treatmentc | No. of cells | Unbudded cells (%) | Cells with bud (%) | Shmoos (%) |
White | ESP | 0 | − | 276 | 70 | 30 | 0 |
8 | − | 289 | 17 | 83 | 0 | ||
8 | + | 213 | 7 | 93 | 0 | ||
White | LSP | 0 | − | 206 | 97 | 3 | 0 |
8 | − | 204 | 5 | 94 | 0 | ||
8 | + | 214 | 6 | 94 | 0 | ||
Opaque | ESP | 0 | − | 258 | 76 | 25 | 0 |
8 | − | 224 | 22 | 78 | 0 | ||
8 | + | 200 | 9 | 4 | 85 | ||
Opaque | LSP | 0 | − | 221 | 86 | 13 | 0 |
8 | − | 194 | 15 | 85 | 0 | ||
8 | + | 206 | 9 | 19 | 72 | ||
aESP, early saturation phase; LSP, late saturation phase. | |||||||
bWhite or opaque cells were grown at 25°C in supplemented Lee's medium for 36 h to early saturation phase or for 48 h to late stationary phase, and diluted into fresh medium at 2 × 106/ml. Cells were then incubated for 8 h. | |||||||
cFor pheromone-treated cells (+), pheromone was added every hour for 7 h, then cells were harvested at 8 h. Untreated (−) cells were also harvested at 8 h. |