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. 2006 Apr 27;25(10):2263–2273. doi: 10.1038/sj.emboj.7601108

Figure 1.

Figure 1

Eg5 motor proteins. (A) The domain structure of the human gene as predicted by PAIR-COIL with Eg5-367 and Eg5-513. (B) Analytical gel filtration was carried out to compare Eg5-513, Eg5-513-5His, dimeric Kinesin-1 K401, and monomeric Eg5-367. The void volume eluted at 16 min and the included volume at 51.5 min. (C) Gel filtration of Eg5-513 at the protein concentration range used for the reported experiments indicates that the proposed dimeric state of Eg5-513 remained stable over the range of protein concentrations used in this study (0.5–4 μM). The inset shows an SDS–Coomassie blue stained gel of Eg5-513.