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Eg5-513-5His promotes plus-end-directed MT gliding in the presence of 1.5 mM MgATP. Polarity-marked MTs exhibit a more highly fluorescent MT minus-end. ▴ indicates a MT that is changing its position over time in comparison to the stationary MT denoted by ★. Conventional Kinesin-1 (KHC) was used to assess the polarity of the MTs. See Supplementary data for a movie.
