Table 1.
Transactivation phenotypes of previously characterised Tax mutants in Jurkat cells
| Mutation | LTR | NFkB | ||
| Mo | WT | None | 100% | 100% |
| M22 (S130A/L131F) | 105% | < 10% | ||
| M47 (I319R/L320S) | < 5% | 130% | ||
| 2B | WT | None | 100% | 100% |
| M22 (S130A/L131F) | 110% | < 5% | ||
| M47 (I319R/L320S) | < 5% | 115% |
Jurkat cells were co-transfected with 250 ng of the indicated Tax 2 plasmids, 1 ug of the LTR or NFkB luciferase reporters and 50 ng of the control Renilla plasmid pRL TK. After 24 hrs, the cells were lysed and reporter activities were measured using a Turner 20/20 Luminometer. Firefly reporter activities were normalised using Renilla luciferase values. Normalised values for mutant Tax proteins were calculated as a percentage of the wildtype Mo or Tax 2B values which was set at 100%.