Table 4.
Transactivation phenotypes of Tax 2B mutants
| Mutation | LTR | NFkB | |
| 2B | None | 100% | 100% |
| G21D | 26% | 138% | |
| L87I | < 10 % | 90% | |
| P92L | 24% | 86% | |
| Y144C | 58% | 65% | |
| Y144R | < 5% | < 5% | |
| A204T | 41% | 54% | |
| W248R | < 5% | < 5% | |
| L308V | 66% | 88% | |
| Mo | None | 41% | 73% |
Jurkat cells were co-transfected with either wildtype Tax 2B or Tax 2B mutants together with 1 ug of the LTR or NFkB luciferase reporters and 50 ng of the control Renilla plasmid pRL TK. After 24 hrs the cells were lysed and reporter activities were measured using a Turner 20/20 Luminometer and firefly reporter activities were normalised using Renilla luciferase values. Normalised values for wildtype Mo and mutant Tax proteins were calculated as a percentage of wildtype 2B values which was set at 100%.