Skip to main content
. 2006 May 19;2(5):e71. doi: 10.1371/journal.pgen.0020071

Figure 2. Drosophila Nbs Is Required for Damage-Induced Cell Cycle Arrest.

Figure 2

Third-instar larval wing discs were mock treated or treated with various doses (250, 500, 1,000, and 4,000 rads) of X-rays and then stained with an antibody against phosphorylated histone H3.

(A–I) The pattern of mitotic cells in untreated and irradiated wild-type (A–C), nbs mutant (D–F), and tefu mutant (G–I) larval wing discs are shown. At 1,000 and 4,000 rads, mitosis is blocked in wild-type wing discs (B and C) whereas nbs mutant discs fail to arrest (E and F). tefu mutant wing discs have a partial mitotic arrest following treatment with 1,000 rads (H). At 4,000 rads, mitosis is completely blocked in tefu mutant wing discs (I).

(J) The ratio of mitotic cells in wild-type, nbs, tefu, and mei-41 mutant wing discs following X-irradiation to the number of mitotic cells in untreated discs of the same genotype is shown. Error bars indicate the standard error of the mean.