Figure 5.

Allele-specific methylation in H19-associated DMR in CRCs with ROI and LOI of IGF2. (A) Methylation status in the DMR of the six CRCs with ROI by bisulfite-sequencing. Each line represents a separate clone. Methylated CpGs are shown as filled beads and unmethylated ones as open beads. One allele is fully methylated, and the other allele is low-level methylated, indicating uniform allele-specific methylation in CRCs with ROI. (B) Methylation status in the DMR of the six CRCs with LOI. One allele is methylated fully, whereas the other allele is methylated partially. All samples with LOI showed the boxed CTCF-binding site (CpG sites 5–9 indicated in Fig. 4) to be methylated in both alleles.