Figure 3.

PKC function is necessary for the differentiation of thymocytes to the CD4⁺ CD8⁺ stage. Intact fetal thymic lobes from d14 timed-pregnant CD1 mice were retrovirally infected with either (A) vector alone (MIEV) or (B) PKC-KR encoding constructs for 72 h in HOS-FTOC, then incubated in standard FTOC for 5 days. Flow cytometric analysis of CD4 vs. CD8 cell surface expression on thymocytes gated for GFP expression is shown (R1, GFP⁻; R2, GFP^int; R3, GFP^hi). The data shown are representative of four independent experiments.