Sites of Adm expression determined by EGFP fluorescence.
(a) Frozen section of adrenal gland from an adult
Adm+/−. Note intense fluorescence in
adrenal medulla (m) and the absence of fluorescence in adrenal cortex
(c). (b) Confocal microscopy of an E9.5
Adm+/− embryo. Moderate expression is seen
in the heart (arrowhead h), and strong expression in the developing
vasculature (arrowhead v) and forebrain (arrowhead b); a speck of
nonspecific fluorescence has been covered with a black box.
(c) Frozen section of an E13.5
Adm+/− embryo, showing expression in the
developing left ventricle. Arrowheads indicate the pericardial surface
of the left ventricle. (d) Frozen section of an E16.5
Adm+/− embryo, showing expression in the
aorta (a), esophagus (oe), and thoracic duct (td).