Figure 2.

Polymerization of fibrinogen by anastellin. Anastellin and fibrinogen at various concentrations were mixed 1:1 (vol/vol), and protein polymerization was followed by monitoring optical density at 620 nm (turbidity). (A) An increase in turbidity is seen when a constant amount of fibrinogen (5 mg/ml) is mixed with increasing amounts of anastellin. (B) A constant amount of anastellin (3 mg/ml) was mixed with an increasing amount of fibrinogen. Mixing fibrinogen with a control fragment from the 11th type III repeat of fibronectin (III-11C; 3 mg/ml) yielded essentially baseline turbidity (data not shown).