Figure 3.

Inhibition of KRIB osteosarcoma growth by anastellin, sFN, and sFBG. s.c. tumors were grown in nude mice from KRIB osteosarcoma cells. Treatment by i.p. injections of the indicated substances (six mice per treatment group) were started 3 weeks after tumor implantation, repeated twice a week, and continued for 5 weeks. Each injection consisted of 100 μg of fibronectin mixed with 300 μg of anastellin (sFN), 500 μg of fibrinogen mixed with 300 μg of anastellin (sFBG), or 600 μg of anastellin. Tumor sizes (mean and SEM) in each treatment group (six mice per group) at the indicated time point are shown. The tumors of the mice treated with anastellin, sFN, or sFBG were significantly smaller at the end of the experiment than those of the mice treated with vehicle alone (anastellin, P < 0.01; sFN, P < 0.05; sFBG, P < 0.01).